超氧化物化学发光探针底价供应

本产品仅供科研实验，不得用于医疗或食用。本公司今日报道:

超氧化物化学发光探针底价供应

产品名称：超氧化物化学发光探针
其它名称：Cypridina Luciferin methoxy-analogue, MCLA
英文名称：Polysciences Polyethylenimine HCl MAX, Linear, Mw 40,000(PEI 40,000)
CAS号：162558-52-3
规格：1g
分类：超氧化物化学发光探针
储存条件：请参照产品标签

产品特性 1）CAS NO.：162558-52-3 2）同义名：2-Methyl-6-(4-methoxyphenyl）-3,7-dihydroimidazo[1,2-a]pyrazin-3(7H）-one hydrochloride, 6-(4-Methoxyphenyl）-2-methyl-3,7-dihydroimidazo[1,2-a]pyrazin-3(7H）-one hydrochloride, Cypridina Luciferin methoxy-analogue, MCLA 3）分子式：C14H13N3O2· HCl 4）分子量：291.73 6） Emmax：~465 nm (Chemiluminescence: in 0.1 M Tris pH 8.5; after addition of H2O2） 8）溶解性：溶于水，DMSO，DMF 9）化学结构式：保存：-20ºC避光干燥保存，至少1年有效。使用方法（For reference only） Buffer examples: for Cytochrome C: 50 mM Tris-Cl, pH 7.8, 0.1 mM EDTA, 7 mM sodium succinate . for mitochondria proteins: 125 mM KCl, 10 mM HEPES, 5 mM MgCl2, 2 mM K2HPO4 - Conduct reaction in 100 µl of reaction buffer - Light emission is detected ａnd quantified using a microplate luminometer with opaque (white） 96-well plates. The photomultiplier can be set to default with an integration time of 1000 ms. The MCLA signal is quantified as an integral of 20 s to 60 s of continuous measurement. - A positive control can be prepared with the xanthine/xanthine oxidase system: 1 mM xanthine + 0.1 unit/ml xanthine oxidase causes a ~100-fold increase in chemiluminescent signal as compared with xanthine or xanthine oxidase alone, which did not differ significantly from reaction buffer alone. Addition of SOD (CuZn-SOD from erythrocytes） decreases the MCLA signal by over 98%.





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